Composite
Part:BBa_K2824009:Design
Designed by: Jiang Qiaochu Group: iGEM18_NEU_China_B (2018-10-06)
LsrA promoter-GFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 897
Design Notes
In fact, the complete replication of the six genes that began with LsrA, that is, from LSRA to LSRG, is also a feasible method of construction. Only the single promoter has been able to recognize the signal molecule AI-2, so this construction is relatively simple and convenient.
Source
The promoter of LsrA is from the genome of Escherichia coli K12. GFP gene was a commercial components which is a green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP .